Application of LC/MS – IT – TOF for Screening of WADA Prohibited Doping Peptides
October 24, 2019
Sample Pretreatment for Confirmation of 19 – Norsteroids by GC – C – IRMS in Doping Control
October 24, 2019

Urinary Luteinizing Hormone (LH) Levels in Male Athletes during 3 – Years Routine Doping Control Analysis at the NDCC Bangkok

Categories
Tags

Figure 1 : Number of sample at different LH concentrations in total urine samples analyzed during 2014 -2016.

Urinary Luteinizing Hormone (LH) Levels in Male Athletes during 3 – Years Routine Doping Control Analysis at the NDCC Bangkok

National Doping Control Centre, Mahidol University

Title :

Urinary Luteinizing Hormone (LH) Levels in Male Athletes during 3 – Years Routine Doping Control Analysis at the NDCC Bangkok

Researchers :

Asawesna C., Nimsoongnern S., Intong T., Seetapun S., Kusamran T., Kongpatanakul S.

Luteinizing hormone (LH) is a peptide hormone produced by gonadotropic cells from the pituitary gland, and stimulates production of endogenous testosterone by testicular Leydig cells. The LH secretion is suppressed, by negative control mechanism, with high levels of testosterone in the circulating blood. The LH hormone has been included in the World Anti-Doping Agency (WADA) Prohibited List in ClassS.2 as a peptide hormone whose use is prohibited only for male athletes. This study is aimed to provide statistical evaluation of urinary LH levels from male athletes in sport doping control samples determined during January 2014 – December 2016 at the National Doping Control Centre (NDCC), Thailand. A total of 5,699 urine samples were analyzed by IMMULITE® 1000 LH Chemiluminescent Immunometric Assay Kit (Siemens Healthcare, USA) using the Multi-Level Lyphochek® Immunoassay Plus (BIO-RAD, USA)as the assay control. The in-house validation method has a precision of 4.69 %RSD with the limit of detection (LOD) at 0.15 IU/L. The mean LH concentration from the total urine samples analyzed was 10.34 (range 0.06 – 166.67) IU/L from which lower than 1IU/L and higher than 50 IU/L were found in 423 (7.42 %) and 24 (0.52 %) samples, respectively. Furthermore, 15 samples were found to contain LH concentration over 60 IU/L with the numbers declining from 9 (0.36 %) to 5 (0.28 %) and 1 (0.07 %) during 2014, 2015 and 2016 respectively, with no indication of unusual steroid profile. The concentrations of T, E and the T/E ratios of all samples were not significantly out of the WADA guideline normal ranges and showed no correlation with the urinary LH levels. The results provided an additional information for further considering of the ATF findings for the hormone level.

Figure 1 : Number of sample at different LH concentrations in total urine samples analyzed during 2014 -2016.
Table 1 : LH concentratiohs (IU/L) from 5,699 urine samples including in competition (IC) and out of competition (OOC) analyzed at the NDCC during 2014 - 2016.
Table 2 : Number of samples containing various intervals of LH concentrations according to dif'ferent WADA guidelines.
Table 3 : T, E concentrations (ng/mL) and T/E ratios of samples containing LH levels over 50 IU/L. Data from 2015 - 2016. * Value less than 1 ng/mL.

Publishing :
Recent Advances in Doping Analysis (25) Proceeding of the Manfred Donike Workshop 35th Cologne Workshop on Dope Analysis. 5th to 10th March 2017, page 148-151.

Key Contact Person :
Mrs.Morakot Kaewklum
National Doping Control Centre, Mahidol University
+66 2 354 7149 – 50